Detect disease-relevant regions quickly and reliably

Fluorescence in-situ hybridization combines the methods of classical cytogenetics and molecular genetics. It is therefore also referred to as molecular cytogenetics. With FISH, gene or chromosome abnormalities can be detected directly in tissues, cell nuclei or chromosomes. Specific, synthetically produced fluorescence dye-labelled oligonucleotides serve as probes for the detection of the smallest chromosome regions, which can be analyzed with a fluorescence microscope. The method of fluorescence in-situ hybridization can be performed on metaphase chromosomes and interphase nuclei. A special method in prenatal diagnostics is the so-called prenatal rapid test. Using this special FISH analysis, uncultivated amniotic cells can be examined for numerical aberrations of chromosomes 13, 18, 21, X and Y within one day.

FISH analyses are useful:

  • for the exclusion of microdeletions and other chromosomal aberrations (metaphase-FISH)
  • for rapid detection of the most common trisomies 13, 18 and 21 as well as sex chromosomes (interphase-FISH / prenatal rapid test)
  • for the detection of leukemia-specific chromosomal aberrations (tumor-FISH)

Please find the request form for our "Interdisciplinary Blood Cancer Diagnostics" as well as the list of our FISH probes here: 

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Molecular cytogenetics (FISH)

Material TAT (after sample receipt)
Metaphase-FISH prenatal    
  Amniotic fluid Approx. 10 - 15 ml (discard the first 2 ml), storage and transport at room temperature.
Never centrifuge!
Only perform together with a conventional chromosome analysis.
8 - 10 days
  Chorionic villi (CVS)

approx. 20 - 30 mg, use sterile transport medium*.

Only perform together with a conventional chromosome analysis.
Culture: 8 - 10 days
  postnatal     
  Blood Lithium-Heparin-Blut:
Neugeborene:                  1 - 2,5 ml
Kinder & Erwachsene:   5 - 7 ml
5 - 10 days
Interphase-FISH prenatal     
Prenatal rapid test Amniotic fluid In addition to conventional chromosome analysis, take 3 ml of amniotic fluid. Not possible for bloody amniotic fluid samples. Only to be performed together with conventional chromosome analysis. 1 - 3 days
  postnatal aus    
  Blood Lithium heparin blood: newborns: 1 - 2.5 ml 1 - 3 days
Tumor-FISH      
  Bone marrow (Minimum of 10 x 106 mononuclear cells) 3 - 5 ml heparinized bone marrow aspirate. If cytogenetic analysis is also required:
5 - 10 ml heparinized bone marrow aspirate.
8 - 10 days
  Leucemia blood (amoutn of blasts ≥ 20 %) 3 - 5 ml heparinized peripheral blood. If a cytogenetic analysis is also required:
5 - 10 ml heparinized peripheral blood.
8 - 10 days

* Available via our local agents; alternatively a sterile-coated tube with physiol. NaCl and antibiotics (Penicillin 100 µg/ml, Streptomycin 100 µg/ml or Gentamycin 50 µg/ml) can be used.

 

"I will be glad to answer your questions about our molecular cytogenetic offer."

Contact me: anja.kron@bioscientia.de or +49 6132 781 240
Dipl. Biologist Anja Kron, Head of Cytogenetics
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