Detect disease-relevant regions quickly and reliably
Fluorescence in-situ hybridization combines the methods of classical cytogenetics and molecular genetics. It is therefore also referred to as molecular cytogenetics. With FISH, gene or chromosome abnormalities can be detected directly in tissues, cell nuclei or chromosomes. Specific, synthetically produced fluorescence dye-labelled oligonucleotides serve as probes for the detection of the smallest chromosome regions, which can be analyzed with a fluorescence microscope. The method of fluorescence in-situ hybridization can be performed on metaphase chromosomes and interphase nuclei.
FISH analyses are useful:
- for the exclusion of microdeletions and other chromosomal aberrations (metaphase-FISH)
- for the detection of leukemia-specific chromosomal aberrations (tumor-FISH)
Please find the request form for our "Interdisciplinary Blood Cancer Diagnostics" as well as the list of our FISH probes here:
|
Molecular cytogenetics (FISH) |
Material | TAT (after sample receipt) | |
| Metaphase-FISH | prenatal | ||
| Amniotic fluid | Approx. 10 - 15 ml (discard the first 2 ml), storage and transport at room temperature. Never centrifuge! Only perform together with a conventional chromosome analysis. |
8 - 10 days | |
| Chorionic villi (CVS) |
approx. 20 - 30 mg, use sterile transport medium*. Only perform together with a conventional chromosome analysis. |
Culture: 8 - 10 days | |
| postnatal | |||
| Blood | Lithium-Heparin-Blut: Neugeborene: 1 - 2,5 ml Kinder & Erwachsene: 5 - 7 ml |
5 - 10 days | |
| Interphase-FISH | postnatal | ||
| Blood | Lithium heparin blood: newborns: 1 - 2.5 ml | 1 - 3 days | |
| Tumor-FISH | |||
| Bone marrow (Minimum of 10 x 106 mononuclear cells) | 3 - 5 ml heparinized bone marrow aspirate. If cytogenetic analysis is also required: 5 - 10 ml heparinized bone marrow aspirate. |
8 - 10 days | |
| Leucemia blood (amoutn of blasts ≥ 20 %) | 3 - 5 ml heparinized peripheral blood. If a cytogenetic analysis is also required: 5 - 10 ml heparinized peripheral blood. |
8 - 10 days | |
* Available via our local agents; alternatively a sterile-coated tube with physiol. NaCl and antibiotics (Penicillin 100 µg/ml, Streptomycin 100 µg/ml or Gentamycin 50 µg/ml) can be used.
