Molecular genetics

Postnatal

 
Material-Options Volume TAT (after sample receipt):

EDTA blood (room temperature)

Infants:                         1-2 ml

Children & adults:  3-5 ml

Please contact us

 

DNA* mind. 1-5 µg Please contact us

*Nucleic acid will only be accepted as a specimen type, if extraction is performed in a CLIA-certified laboratoty or a laboratory that meets equivalent requirements.

Prenatal

After previous consultation.

Important: Send 3-5 ml EDTA blood from the mother for maternal contamination exclusion. 

Material-Options Volume TAT (after sample receipt):

fetal DNA*

mind. 1-5 µg

Please contact us

 

Amniotic fluid 20 ml Please contact us
Chorionic villi (CVS) mind. 10-20 mg in transport medium Please contact us

*Nucleic acid will only be accepted as a specimen type, if extraction is performed in a CLIA-certified laboratoty or a laboratory that meets equivalent requirements.

 

Array-CGH

Prenatal:

Important: EDTA- / Heparin-blood of the mother for a maternal contamination exclusion or for a prompt further examination with unclear copy number variants.

 

Material Volume TAT (after sample receipt):
Amniotic fluid 20 ml approx. 10 days (after harvesting of cells from cell culture)
chorionic villi 
for chromosome and array CGH analysis
min. 30 mg approx. 10 days (after harvesting of cells from cell culture)

 

Postnatal:

Material Volume TAT (after sample receipt):
EDTA-Blood  (room temperature)

Infants: 1-2 ml

Children & Adults: 3-5 ml

4 - 8 weeks

 

Cytogenetics

Chromosome analyses: prenatal

Material Volume TAT (after sample receipt)
Amniotic fluid Approx. 10 - 15 ml amniotic fluid (discard the first 2 ml), store and transport at room temperature.Never centrifuge! 8 - 10 days
Chorionic villi (CVS) Approx. 20 - 30 mg chorionic villi, for transport use sterile medium*. Direct preparation: 1 - 2 days
Culture: 8 - 10 days
Cord blood 2 - 3 ml umbilical cord blood in Lithium Heparin tubex. 7 days
Product of conception (POC) Chorionic villi, parts of umbilical cord, Achilles tendon, fascia lata. Use sterile transport medium*.Do not give in formalin! 10 - 14 days

* Available via our local agents; alternatively a sterile-coated tube with physiol. NaCl and antibiotics (Penicillin 100 µg/ml, Streptomycin 100 µg/ml or Gentamycin 50 µg/ml) can be used.

x To exclude maternal cell contamination, an EDTA blood sample (2-3 ml) is additionally required (Reference shipment).

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Chromosome analyses: postnatal

Material Volume TAT (after sample receipt)
Blood Lithium Heparin blood
Newborns:                             1 - 2.5 ml
Children and adults:          5 - 7 ml
14 - 21 days;
Urgent cases: 7 - 10 days
Fibroblasts Skin biopsy, approx. 5 mg / 2 - 10 mm² of tissue. For transport use sterile medium*.Do not give in formalin! 14 days
Cord blood 2 - 3 ml umbilical cord blood in Lithium Heparin tubex. 7 days
Product of conception (POC) Chorionic villi, parts of umbilical cord, achilles tendon, fascia lata. Use sterile transport medium*.Do not give in formalin! 10 - 14 days

* Available via our local agents; alternatively a sterile-coated tube with physiol. NaCl and antibiotics (Penicillin 100 µg/ml, Streptomycin 100 µg/ml or Gentamycin 50 µg/ml) can be used.

Tumor cytogenetics

Material Volume TAT (after sample receipt)
Bone marrow (Minimum of 10 x 106 mononuclear cells) 5 - 10 ml heparinized bone marrow aspirate 8 - 10 days
Leucemia blood (amoutn of blasts ≥ 20 %) 5 - 10 ml heparinized peripheral blood 8 - 10 days

 

 

Fluoreszenz in-situ Hybridisierung (FISH)

Molecular cytogenetics (FISH)

  Material TAT (after sample receipt)
Metaphase-FISH prenatal    
  Amniotic fluid Approx. 10 - 15 ml (discard the first 2 ml), storage and transport at room temperature.
Never centrifuge!
Only perform together with a conventional chromosome analysis.
8 - 10 days
  Chorionic villi (CVS) approx. 20 - 30 mg, use sterile transport medium*.
Only perform together with a conventional chromosome analysis.
Culture: 8 - 10 days
  postnatal    
  Blood Lithium-Heparin-Blut:
Infants: 1 - 2,5 ml
Children & Adults: 5 - 7 ml
5 - 10 days
Interphase-FISH prenatal    
Prenatal rapid test Amniotic fluid In addition to conventional chromosome analysis, take 3 ml of amniotic fluid.
Not possible for bloody amniotic fluid samples.
Only to be performed together with conventional chromosome analysis.
1 - 3 days
  postnatal    
  Blood Lithium heparin blood:
newborns: 1 - 2.5 ml
1 - 3 days
Tumor-FISH      
  Bone marrow (Minimum of 10 x 106 mononuclear cells) 3 - 5 ml heparinized bone marrow aspirate.
If cytogenetic analysis is also required: 5 - 10 ml heparinized bone marrow aspirate.
8 - 10 days
  Leucemia blood (amount of blasts  ≥ 20 %) 3 - 5 ml heparinized peripheral blood.
If a cytogenetic analysis is also required: 5 - 10 ml heparinized peripheral blood.
8 - 10 days

* Available via our local agents; alternatively a sterile-coated tube with physiol. NaCl and antibiotics (Penicillin 100 µg/ml, Streptomycin 100 µg/ml or Gentamycin 50 µg/ml) can be used.